Basic course cryosectioning and Immuno-gold labeling of thawed frozen sections (Tokuyasu technique)

The workshop is primarily practical in nature, although some topics will be dealt with in lectures. The entire procedure from (live) specimen till immuno-labeled ultrathin section will be covered from start to finish. Most of the time will be spent in the lab working with standard specimens like cultured cells or tissues. Besides managing the ultra cryo-microtome, the preparation of tools and other necessities will be covered.

Date: Jan 23-26, 2015

Target audience: The workshop is meant for people that do not have experience with the Tokuyasu technique. Experience in resin sectioning is not necessary, but is definitely an advantage.

Program: See course website.

Course website: