In this workshop, a general background to metagenomics is provided. The workflow of sample preparation, DNA isolation, platform selection, read mapping, annotation and metagenome assembly is covered. Attention is paid to taxonomic classification, quantification of microbial communities and species richness. In addition, functional properties of the microorganisms in the samples are reviewed. Pipelines for data analysis and presentation of the data are discussed.
Hands-on data analysis is an important part: during practical sessions, you will get to analyse both 16S and metagenome data sets.
This workshop is intended for laboratory professionals who are involved in metagenomics research but who have limited experience with the relevant techniques or who are outsourcing their metagenomics analysis.