Microscopy and Spectroscopy in Food and Plant Sciences

The course covers a range of advanced microscopic and microspectroscopic techniques (see below), that have a wide range of applications in both the Food and Plant Sciences.

Date: May 6-9, 2014

Target audience: PhD students.

Program: The following topics will be treated during the lectures (in the morning) with examples of application in food and plant sciences: Microscopy, fluorescence, confocal microscopy, Förster Resonance Energy Transfer (FRET) imaging, Fluorescence Lifetime Imaging Microscopy (FLIM), reporter imaging/ image analysis, 3D Imaging, Fluorescence Recovery After Photobleaching (FRAP), time- and spectrally-resolved fluorescence spectroscopy, total internal reflection fluorescence (TIRF), spinning disk microscopy, differentical interference contrast microscopy (DIC), streak-camera, time-correlated single-photon counting (TCSPC), fluorescence anisotropy and circular dichroism (CD).

Four practicals of each 2 hours will be given for all PhD students (groups of 4 students per setup) on Tuesday and Wednesday afternoon. On Thursday the students do a more elaborate experiment of choice, preferably applying one of the afore-mentioned techniques to their own samples and the results and interpretation will be presented and discussed on Friday afternoon.

Course website: http://www.vlaggraduateschool.nl/courses/mic-spec.htm