NGS: RNA-seq data analysis

The BioSB research school and partner LUMC are organizing a 3-day course on RNA-seq data analysis. This is an advanced course for people with experience in NGS. The course will consist of seminars and hands-on command line, Galaxy and R practicals and will cover the analysis pipelines for differential transcript expression and variant calling. Examples will be taken from human and mouse studies. The course does not cover prokaryotic RNA profiling nor plant- and metagenomics aspects.

Date: November 6-8, 2017

Target audience:
Participants for the RNA-seq course should preferably have participated in the general NGS course or otherwise have demonstrated hands-on experience with NGS data analysis. The course is aimed at PhD students and post-docs, but scientific programmers and data analysts with a background in biology and bioinformatics may also attend. Registrations will be handled on a first-come-first-served basis but experience and motivation will be reviewed. Seats have been reserved for participants in the FP7-funded project RD-Connect and Marie Curie European Training Network TranCYST.


Course topics:

  • RNA-seq experimental approaches
  • Alignment and de novo assembly
  • Statistics for differential gene expression
  • eQTL analysis and allele specific expression
  • Variant calling and RNA editing
  • Fusion transcript detection
  • Small RNA profiling
  • Software for RNA-seq data analysis

Keywords: Next-generation sequencing; NGS; data analysis; RNA-seq; transcriptome

Course website: