This short course aims at training the participant in the basic principles and applications of the Polymerase Chain Reaction (PCR) and real-time PCR. The program consists of a series lectures and experiments covering all basic PCR topics like efficiency, fidelity and specificity of the PCR reaction.
Date: April 7, 2015
Target audience: This course is developed as an introduction to the PCR and especiall suitable as a basic introduction to our advanced course on Real-time PCR in molecular diagnostics (2548). Basic knowledge of molecular biology in general is desirable.
Program:
Theory:
Principles of DNA amplification technologies; The biochemical and physical aspects of the PCR
Principles, parameters, and design of (real-time) PCR primers
Principles and parameters of detection of PCR products
Principles of Real-time PCR; detection formats, assay formats
PCR contamination prevention
Practicals:
Real-time PCR dilution series and melting curve analysis:
Optimization and determination of the sensitivity of real-time PCR with SybrGreen
Optimization and determination of the sensitivity of real-time PCR with Hybridisation probes
Course website: http://www.alsavans.nl/avans-training-2545.htm
