Proteomics is the study of the proteome, the set of proteins expressed in an organism. The proteome can change dramatically depending on biological conditions. Proteomics is able to capture detailed snapshots of the proteome using mass spectrometry techniques. The rapid development in technical advances of instrumentation has led to new bioinformatics approaches. In this course the focus will be on sample preparation and data analysis.

Date: April 27-May 1, 2015

Target audience: PhD students or Postdocs interested in this course should work on proteomics projects or plan to use proteomics in the near future and have a strong background in nutrition, biochemistry, physiology, molecular biology or a comparable discipline.

Program: The course is a 4 days hands-on course. In the morning sessions lectures on mass spectrometry and proteomics applications and in the afternoon sessions practical work are scheduled. Participants can bring their own sample and one slice of a gel (1D gel is preferred, but 2D gels are also possible, advise on the selection criteria will be provided) will be measured using splittless nano-HPLC Orbitrap FTMS.
Sample preparation protocols
Quantitative techniques
Protein complexes
MaxQuant and Perseus
Integration of ~omics data
New mass spectrometric tools in proteomics

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