Amsterdam UMC – PhD student – Local immunometabolites shape inflammatory macrophages and atherosclerosis progression

Your work environment
For this collaborative project funded by Amsterdam Cardiovascular Sciences (ACS), we seek a highly motivated researcher with affinity for cardiovascular biology, metabolism and/or immunology.

This translational project incorporates fundamental research questions as well as translational applications in in vivo models, human in vitro models and analysis of atherosclerosis patient material with the ultimate goal to translate our findings to the clinic. To do so, we integrated our complementary expertise on macrophage immunometabolism (Jan Van den Bossche), atherosclerosis (Prof. Menno de Winther) and metabolomics (Michel van Weeghel) to create a unique research environment to successfully perform this interdisciplinary research.

This joint project will be performed at Amsterdam UMC in the Department of Molecular Cell Biology and Immunology (location VUmc; O|2 Lab Building) , in the Department of Medical Biochemistry, and in Laboratory of Genetic Metabolic Diseases (both location AMC). At these places, you will work in a team with young and enthusiastic researchers, who have expertise in immunology, biochemistry, cell biology, analytical chemistry, cardiovascular disease and cancer. Our ultimate goal is to discover new metabolic targets for treatment and biomarkers for diagnosis.

Recent relevant publications:
Van den Bossche J, Baardman J, Otto NA, van der Velden S, Neele AE, van den Berg SM, Luque-Martin R, Chen HJ, Boshuizen MC, Ahmed M, Hoeksema MA, de Vos AF, de Winther MP. Mitochondrial Dysfunction Prevents Repolarization of Inflammatory Macrophages. Cell Reports. 2016 Oct 11;17(3):684-696.Van den Bossche J, O’Neill LA, Menon D. Macrophage Immunometabolism; Where Are We (Going)? Trends in Immunology, 2017 Jun;38(6):395-406.

Your challenge
The successful candidate will study the immunometabolic alterations in human plaque-associated macrophages and will address the importance of this process with both in vitro tools and in vivo models for atherosclerosis.
As a junior researcher, your main tasks and responsibilities are:

  • Integrating metabolomics data to define immunometabolic circuits in atherosclerotic plaque macrophages;
  • Identifying new metabolic regulators that control macrophage function;
  • Validating new targets in mouse models of atherosclerosis;
  • You will use state-of-the-art high-dimensional cytometry techniques, RNA-sequencing, mass spectrometry, and will apply bioinformatics to analyze the data.

Your profile
We are looking for a highly motivated and enthusiastic researcher with:

  • A Master’s degree in a field related to biomedical sciences, (medical) biology, biochemistry, metabolism or related field;
  • Affinity for immunology, metabolism and cardiovascular biology;
  • Interest in high-dimensional technologies and pushing the boundaries of research through technology-driven applications;
  • Experience with animal experiments (article 9 qualified), high-dimensional data analysis and biostatistics is a plus;
  • Excellent communications skills in English, both written and verbal.

Read more