UvA – PhD candidate in Molecular Cell Biology

The Swammerdam Institute for Life Sciences (SILS) is one of the largest institutes of the Faculty of Science. Its approximately 240 scientists and staff members work in 16 research groups that perform excellent research centered on three themes:

  • Cell & Systems Biology;
  • Neurosciences, and
  • Molecular Life Sciences.

The research performed in the section Molecular Cytology focusses on ‘Self-organization and signaling in living cells’. In this context we are seeking for an enthusiastic and talented PhD candidate who likes to work in a multi-disciplinary team of scientists.

Project description
The research project aims at engineering novel genetically encoded fluorescent probes with lifetime contrast that can be used for functional imaging of cellular processes. This project builds on our extensive experience in the field of fluorescent protein engineering, signal transduction and functional imaging. Due to recent developments in imaging technology it is possible to obtain fluorescence lifetime maps with high temporal resolution. This enables true quantitative imaging of molecular processes in living cells. To expand the application of this technology, we will generate a new class of genetically encoded fluorescent probes that display lifetime contrast.

The proposed project involves biochemistry (protein purification, spectroscopic characterization), molecular biology (cloning, generation of DNA libraries), eukaryotic cell culture, fluorescent biosensor engineering, high content in vivo screening and advanced microscopy.

The project is funded by a grant from the Netherlands Organization for Scientific Research (NWO).

Requirements
Candidates should have a Master’s degree in Biochemistry, Cell biology, or Medical biology (or equivalent), have excellent communication skills and be highly motivated to conduct fundamental scientific research in a team of enthusiastic scientists. Experience with fluorescence microscopy, or protein engineering is a plus.

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