Science Park 104 1098 XG Amsterdam
Dr. T.S. Shimizu
0031 (0)20 7547242
The Biomolecular laboratory is a facility for preparation and characterization of biological samples.It is a fully equipped wet lab which provides facilities for protein purification, bacterial cell culture and mammalian cell culture. It contains the following facilities: ultracentrifuges, chromatography system, recombinant expression in bacteria, fluorescence plate reader, incubators and shakers, PCR, FACS and rheometers. In this laboratory, the following optical and imaging techniques are available: total internal reflection (TIRF) and epi-fluorescence microscopy, spinning disk and two-photon confocal fluorescence microscopy, video-enhanced differential interference contrast (DIC) microscopy, coherent anti-stokes raman (CARS) microscopy, in-vivo single molecule imaging, fluorescence correlation spectroscopy (FCS), optical tweezers and micro-rheology.
Further information can be found here: https://amolf.nl/research/systems-biophysics-program
- Biomedical & health
- Single molecule microscopy
- Mathematical modelling of biological systems
- Cellular biophysics
- Systems biology
Expertise and Track Record
At AMOLF, we develop new custom techniques and approaches for quantitative 3D microscopy of multicellular systems, relying on extensive in-house technical support to design and construct microscopy setups. Using our local clean room facilities, we can interface those microscopy setups with microfluidic devices, to precisely control and perturb the chemical environment of our biological system of interest. In addition, we develop novel image and data analysis approaches to extract quantitative information, such as cell positions, cell shape, cell divisions, gene expression levels and subcellular markers, from microscopy images.
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– The Tans group has developed methods to accurately measure growth rates of single cells at sub-cell-cylce resolution (Kiviet et al., Nature 2014).
– The Shimizu and Tans groups have developed a novel technique to track the 3D movement of freely moving bacterial cells (Taute et al., 2015, Nature Communications 6:8776)
– The Van Zon group has developed a novel approach that combines custom microscopy with microfabricated chambers, to follow the dynamics of single cells during the entire ~40 hour post-embryonic development of C. elegans in freely moving larvae (Gritti et al., 2016, Nature Communications 7:12500).
- Laser-scanning confocal microscopy (Nikon Ti2 + AR1)
- Superresolution microscopy (Custom-made)