University Medical Center Utrecht
Heidelberglaan 100 3584 CX Utrecht
Dr. G. Posthuma
0031 (0)88 7556548
With the plethora of molecular information coming from genetic and proteomic studies, the demand for functional analysis of the identified gene products is overwhelming. The validity of microscopy-based methods for functional studies is that single cells and even single organelles can be analyzed, which yields unique and indispensable information for understanding molecular function within the context of the cell. Protein location is an important parameter for protein function and structure is unequivocally linked to function. When microscopy-based research is combined with genetic, proteomic, molecular and biochemical approaches, a full physiological ‘picture’ of cell life will ultimately be in reach. The Cell Microscopy Center harbors a combination of state-of-the-art microscopy techniques.
This group participates in
NL-BioImaging AM (http://www.eurobioimaging.nl).
Nemi: Netherlands Electron Microscopy Infrastructure (http://www.e-microscopy.nl/)
- Biomedical & health
- Immuno-gold electron microscopy on resin or thawed ultrathin cryo sections (Tokuyasu technique)
- Correlative light and Electron microscopy
- Intracellular vesicle trafficking
- • Lysosomal storage diseases
Expertise and Track Record
43500984157 Advanced CLEM imaging of antibodies targeting low expressing, clinically relevant cancer targets for the development of Antibody Drug Conjugates with J. Klumperman (UMC Utrecht)
The Cell Microscopy core is part of the Cell Biology group at the UMC Utrecht. We have provided the past decades services to local (Utrecht), national (Netherlands) and international research groups both public institutions and industry. Depending on the underlying scientific question the support ranges from routine electron microscopical techniques to advanced correlative light and electron microscopy (CLEM) techniques.
The CMC organizes on a yearly basis the following courses:
· Basic course for resin embedding and sectioning
· Basic course immuno- gold electron microscopy (Tokuyasu technique)
· Advanced course for Correlative light and electron microscopy in which both life cell CLEM and Section Clem are covered.
· Immuno electron-microscopical localisation and quantification of antimicrobial cathelicidins.
· Sterelogical measurements of intracellular changes in BAR-T cells after drug administration.
· Description of bile canaliculus lumen changes in ATB8B1 mutated mice.
- Schneider, V. A. F., Coorens, M., Ordonez, S. R., Tjeerdsma-van Bokhoven, J. L. M., Posthuma, G., van Dijk, A., … Veldhuizen, E. J. A. (2016). Imaging the antimicrobial mechanism(s) of cathelicidin-2. Scientific Reports, 6, 32948. http://doi.org/10.1038/srep32948
- Verbeek, R. E., Siersema, P. D., Vleggaar, F. P., ten Kate, F. J., Posthuma, G., Souza, R. F., de Haan, J., van Baal, J. W P M (2016) Journal of Gastrointestinal and Liver Diseases, volume 25, issue 3, pp. 273 - 282
- Bruurs, L. J. M., Donker, L., Zwakenberg, S., Zwartkruis, F. J., Begthel, H., Knisely, A. S., Bos, J. L. (2015). ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization. The Journal of Cell Biology, 210(7), 1055–1063. http://doi.org/10.1083/jcb.201505118
- Zeiss LSM 510, Life cell imaging
- Zeiss LSM 700
- Deltavision RT Core, life cell imaging
- JEOL 1011
- Fei Tecnai 12 , extra features: cryo stage and (cyo) tomography.
- FEI Tecnai 20, extra features: cryo stage and (cyo) tomography
- Leica High pressure freezer
- Leica Ultra (cryo) microtomes