Gemini-Zuid 3.115, Groene Loper 15, 5612 AZ Eindhoven
Prof. dr. K. Nicolay Prof. dr. H. Grüll
0031 (0)40 247 5143
The Center for Biomedical Imaging Research (CIRE) was launched in November 2012, combining the biomedical-imaging infrastructure of Eindhoven University of Technology and Philips Research. CIRE is located at the High Tech Campus in Eindhoven and offers excellent facilities for multimodality imaging research to external partners from academia and industry. CIRE’s world-class facilities include advanced equipment and expertise for Magnetic Resonance Imaging and Spectroscopy (both for research in laboratory animals and human studies), nuclear imaging of laboratory animals (micro-PET and micro-SPECT), micro-CT, optical imaging, minimally invasive MRI-guided interventions, radiochemistry, as well as material research related to contrast agent design and synthesis, and drug delivery. Furthermore, expertise is available on multimodality image analysis and visualization as well as housing and micro-surgical preparation of laboratory animals. In addition, CIRE comprises excellent facilities for cell culture, ex vivo tissue characterization, fluorescence microscopy and immunohistochemistry. CIRE staff offers assistance in experiment design and execution, hands-on training and data analysis.
- Quantitative biomarker imaging
- Multimodality imaging
- Magnetic resonance imaging (MRI)
- Magnetic resonance spectroscopy (MRS)
- High intensity focused ultrasound based therapies
- Preclinical research
- Translational research
- Cardiovascular research
- Metabolic research
- Cancer research
Expertise and Track Record
435000034 Dual-isotope SPECT evaluation of different formats of
nanobodies for tumor imaging with dr. S. Oliveira (UU)
- Van Nierop BJ, Coolen BF, Dijk WJ, Hendriks AD, de Graaf L, Nicolay K, Strijkers GJ. Quantitative first-pass perfusion MRI of the mouse myocardium. Magn Reson Med 2013;69(6):1735-1744. This paper presents, for the first time, robust technology for quantification of perfusion of the mouse heart, based on the measurement of the first-passage of a low-molecular weight Gd-based MRI contrast agent through the different compartments of the heart. Quantification relies on the use of a dual bolus approach to obtain reliable values for both the arterial input function and the myocardial tissue response.
- Bakermans AJ, Geraedts TR, van Weeghel M, Denis S, Joao Ferraz M, Aerts JM, Aten J, Nicolay K, Houten SM, Prompers JJ. Fasting-induced myocardial lipid accumulation in long-chain acyl-CoA dehydrogenase knockout mice is accompanied by impaired left ventricular function. Circ Cardiovasc Imaging 2011;4(5):558-565. This study demonstrates the richness of information that is obtained with high-precision localized 1H-MR spectroscopy of the mouse heart. Here, the MRS tool was employed to assess metabolic and functional changes in mouse myocardium induced by a genetic deficiency in the oxidation of long-chain fatty acids, as a model for an inherited metabolic disorder in man.
- Jonkers RA, van Loon LJ, Nicolay K, Prompers JJ. In vivo postprandial lipid partitioning in liver and skeletal muscle in prediabetic and diabetic rats. Diabetologia 2013;56(3):618-626. This paper highlights the use of the Proton-Observe Carbon-Edited (POCE) MR spectroscopy to assess the dynamics of the incorporation and mobilization of lipids from triglyceride stores in skeletal muscle and liver in relation to the development of insulin resistance and type 2 diabetes. To this aim, uniformly 13C-enriched algal lipid mixture was fed to control, prediabetic and diabetic rats and 13C-enrichment in the intramyocellular lipid pool was quantified with 13C-edited 1H-MRS in a longitudinal study design.
- Kluza E, Jacobs I, Hectors SJ, Mayo KH, Griffioen AW, Strijkers GJ, Nicolay K. Dual-targeting of alphavbeta3 and galectin-1 improves the specificity of paramagnetic/fluorescent liposomes to tumor endothelium in vivo. J Control Release 2012;158(2):207-214. This manuscript deals with MRI-based molecular imaging of angiogenic blood vessels inside mouse tumors with the use of specifically targeted paramagnetic liposomes. It is shown that the specificity of the MRI read-out of angiogenesis targeting is strongly improved with the use of dual-targeted liposomes, which are equipped with peptidic ligands for two tumor endothelial markers.
- De Smet M, Langereis S, van den Bosch S, Bitter K, Hijnen NM, Heijman E, Grull H. SPECT/CT imaging of temperature-sensitive liposomes for MR-image guided drug delivery with high intensity focused ultrasound. J Control Release 2013;169(1-2):82-90. This paper describes a multi-modality imaging study of the tumor delivery of temperature-sensitive drug-loaded liposomes and how this is affected by MRI-guided High-Intensity Focused Ultrasound (HIFU). 111In-labeling of the liposomes was used to allow for quantitatively assessing their biodistribution with SPECT/CT imaging. It was found that tumor hyperthermia with temperatures around 42 oC caused a considerable enhancement of the tumor delivery of doxorubicin-containing thermo-sensitive liposomes.