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Microscopic Imaging – Department of Cell Biology Radboudumc

Contact Details


Geert Grooteplein 28 6525 GA Nijmegen

Prof. dr. P.H.A. Friedl

0031 (0)24 36 55 608

Hotel Description

The Microscopic Imaging Centre (MIC) is specialized in live imaging of tumor and immune cell dynamics by brightfield, fluorescence, fluorescence lifetime, (high content) confocal and multiphoton microscopy. These techniques enables us to image biological processes such as 3D cellular migration in real-time at the subcellular level. Our intravital microscopy platform is integrated within the Preclinical Imaging Centre (PRIME), bridging high-resolution intravital microscopy with state-of-the-art preclinical imaging modalities. Furthermore, infrared multiphoton excitation enables deep tissue penetration, efficient excitation of red fluorescent proteins and label free visualization of tissue matrix structures by Second and Third Harmonic Generation microscopy. The MIC is directed by Prof. dr. Peter Friedl and organized by facility manager Dr. Jack Fransen. This group participates in NL-BioImaging AM (http://www.eurobioimaging.nl). Further information can be found in the link above.

  • Biomedical & health
  • Intravital microscopy
  • Tumor development

Expertise and Track Record

Our facility offers a state-of-the-art intravital microscopy platform, with simultaneous near infrared and infrared excitation and multichannel ultrasensitive emission detection. Thereby, multiple fluorescent proteins can be excited efficiently during long in vivo time lapse measurements. In addition, infrared excitation enables label-free visualization of tissue matrix using second and third harmonic generation microscopy. We also have strong expertise in 3D cell culture and in development and application of the dorsal skinfold chamber animal window model for intravital imaging.

This group participates in Euro-BioImaging (http://www.eurobioimaging.eu/) and in NL-BioImaging AM (http://www.eurobioimaging.nl).

Hotel Characteristics

  • TriMScope II, Lavision Biotec GMbH equipped with three Ti:Sa lasers, OPO (Coherent-APE), Gasp detectors and two X16 FLIM detectors;