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Van Leeuwenhoek Centre for Advanced Microscopy – Molecular Cytology – University of Amsterdam

Contact Details

University of Amsterdam

Science Park 908 1098 XH Amsterdam

http://www.lcam.nl

Prof. dr. T.W.J. Gadella

Th.W.J.Gadella@uva.nl

0031 (0)20 525 6259

Hotel Description

LCAM is a formal collaboration between three innovative microscopy centres at the Faculty of Science (FNWI) of the University of Amsterdam, the Academic Medical Centre (AMC) and the Netherlands Cancer Institute (NKI).
The formalized collaboration capitalizes on a large degree of synergy between the principle investigators in developing, adapting and applying advanced microscopy for use in cell biology. Besides delivering access to the diverse advanced microscopy instruments, LCAM has developed as a leading centre in functional live cell imaging (FRET, FLIM, FCCS, FCS, FRAP and spectral imaging).
This group participates in NL-BioImaging AM (http://www.eurobioimaging.nl).
Further information can be found here: www.lcam.nl

Bioimaging
Public
  • Biomedical & health
  • Agri & Food
  • Industrial biotech
  • Other
  • Functional imaging
  • Super-resolution microscopy
  • Super-resolution reconstruction
  • Cellular signalling

Expertise and Track Record

Advanced super-resolution data analysis

Eurobioimaging pilot exps

  • Ingaramo M, York AG, Hoogendoorn E, Postma M, Shroff H, Patterson GH. Richardson-lucy deconvolution as a general tool for combining images with complementary strengths. Chemphyschem. (2014) 15, 794.
  • Bilan, D.S., Pase, L., Joosen, L., Gorokhovatsky, A.Y., Ermakova, Y.G., Gadella (jr), T.W.J., Grabher, C., Schultz, C., Lukyanov, S. & Belousov, V.V. (2013). HyPer-3: A Genetically Encoded H(2)O(2) Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging. ACS Chem Biol, 8(3), 535-542.
  • Lestini R., Laptenok S.P., Kuhn J., Hink M.A., Schanne-Klein M.-C., Liebl U. and Myllykallio H. Intracellular dynamics of archaeal FANCM homologue Hef in response to halted DNA replication. Nucleic Acid Research 41, 10358-10370 (2013).
  • Bilan, D.S., Pase, L., Joosen, L., Gorokhovatsky, A.Y., Ermakova, Y.G., Gadella (jr), T.W.J., Grabher, C., Schultz, C., Lukyanov, S. & Belousov, V.V. (2013). HyPer-3: A Genetically Encoded H(2)O(2) Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging. ACS Chem Biol, 8(3), 535-542.
  • van der Ploeg R., Verheul J., Vischer N.O., Alexeeva S., Hoogendoorn E., Postma M., Banzhaf M., Vollmer W., den Blaauwen T. Colocalization and interaction between elongasome and divisome during a preparative cell division phase in Escherichia coli. Mol Microbiol. 87:1074-1087 (2013).
  • Lefebvre, B., Klaus-Heisen, D., Pietraszewska-Bogiel, A., Hervé, M., Camut, S., Auriac, M.C., Gasciolli, V., Nurisso, A., Gadella, T.W. & Cullimore, J. (2012). Role of N-glycosylation sites and CXC motifs in trafficking of Medicago trunculata Nod Factor Perception protein to the plasma membrane. The Journal of Biological Chemistry, 287(14), 10812-10823. (2012)
  • Shcherbakova, D.M., Hink, M.A., Joosen, L., Gadella, Th.W.J. & Verkhusha, V.V. (2012). An orange fluorescent protein with a large Stokes shift for single-excitation multicolor FCCS and FRET imaging. Journal of the American Chemical Society, 134(18), 7913-7923.
  • Stetten, D. von, Noirclerc-Savoye, M., Goedhart, J., Gadella, T.W.J. & Royant, A. (2012). Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K. Acta Crystallogr Sect F Struct Biol Cryst Commun, 68(Pt 8), 878-882.
  • Beek, A. ter, Hornstra, L.M., Pandey, R., Kallemeijn, W.W., Smelt, J.P.P.M., Manders, E.M.M. & Brul, S. (2011). Models of the behaviour of (thermally stressed) microbial spores in foods: Tools to study mechanisms of damage and repair. Food Microbiology, 28(4), 678-684.
  • De Vos, W.H., Houben, F., Kamps, M., Malhas, A., Verheyen, F., Cox, J., Manders, E.M.M., Verstraeten, V.L.R.M., Steensel, M.A.M. van, Marcelis, C.L.M., Wijngaard, A. van den, Vaux, D.J., Ramaekers, F.C.S. & Broers, J.L.V. (2011). Repetitive disruptions of the nuclear envelope invoke temporary loss of cellular compartmentalization in laminopathies. Human molecular genetics.

NL-Bioimaging AM & Eurobioimaging (http://eurobioimaging.nl/).: One of the 18 centres participating.
Currently leading two innovative nodes: Super-resolution (Nanoscopy) & Functional imaging

Hotel Characteristics

  • 6 Principle investigators
  • 4 Technicians
  • 1 Labmanager
  • 1 Biostatistican
  • 1 IT specialist

25%

  • FNWI: Confocal: Zeiss LSM510, Nikon A1 (CLEM, FRAP), Olympus FLIM (FLCCS) Spinning Disk Confocal: Zeiss Yokogawa (FLIM), Nikon Andor (TIRF), Zeiss Yokogawa Widefield: Zeiss Cell Observer, Olympus BX, Nikon CLEM

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