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Technology center Flow cytometry – Radboudumc

Contact Details

RadboudUMC

Geert Grooteplein zuid 8, Nijmegen

Harry Dolstra

+31 (0)24 361 97 53

Hotel Description

Flow cytometry is a sophisticated technique of single cell analysis. It simultaneously measures and analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. Some of the characteristics measured include a particle’s relative size, granularity or internal complexity and relative fluorescence intensity.
Area of expertise
•Flow cytometry used in basic and translational research as well as clinical practice and clinical trials.

Services
•Access to flow cytometry analyzers and a flow cytometry sorter
•Advice on multi-color experimental design and data interpretation
•Personal assistance for analysis available by appointment
•Extensive training on analyzers
•Training and education in flow cytometry
•High-end, supervised cell sorting

Phenotyping
Public
  • Biomedical & health
  • • Immunology/Hematology
  • • Genetic and metabolic diseases
  • • Cell biology
  • • Molecular biology
  • • Microbiology

Expertise and Track Record

Services we provide;
•Access to flow cytometry analyzers and a flow cytometry sorter
•Advice on multi-color experimental design and data interpretation
•Personal assistance for analysis available by appointment
•Extensive training on analyzers
•Training and education in flow cytometry
•High-end, supervised cell sorting

Dolstra et al. (2017) Increased Coexpression of PD-1, TIGIT, and KLRG-1 on Tumor-Reactive CD8+ T Cells During Relapse after Allogeneic Stem Cell Transplantation.
Biol Blood Marrow Transplant. 2017 Dec 26
Dolstra et al. (2016) Addition of 10-day Decitabine to Fludarabine/TBI conditioning is feasible and induces tumor-associated antigen specific T-cell responses.
Biol Blood Marrow Transplant 2016 Feb 6.
Woestenenk et al. (2014) Enhanced cellular uptake of albumin-based lyophilisomes when functionalized with cell-penetrating peptide TAT in HeLa cells.
Plos One 2014 Nov 4;9(11)
Dolstra et al. (2011) Concurrent detection of circulating minor histocompatibility antigen-specific CD8+ T cells in SCT recipients by combinatorial encoding MHC multimers.
Plos One 2011;6(6)
Woestenenk et al. (2009) Sorting catalytically active polymersome nanoreactors by flow cytometry.
Small 2009 May;5(10)

Hotel Characteristics

  • 0.2 PhD
  • 1 Operator
  • 0.1 Labmanager

10

  • Beckman Coulter Gallios 10 color
  • Beckman Coulter FC500 5 color
  • BD Aria SORP 18 color sorter (plate sorting 6-384 wells)